Angela Lei
Hello! My name is Angela Lei and I will be a rising senior at the John D. O'Bryant School of Mathematics and Science. I have been going to the O'Bryant for nearly five years, starting in the 7th grade. I am very involved in my school's extracurricular activities. I was an avid participant in my school's Pep Band as well as our Concert Band. I would participate in competitions with other schools, perform in annual school concerts, as well as playing in the Pep Band to show support for my school's athletes. I was also a member and temporary Vice President of my school's Art Club. I joined this club as a means to express myself in any art form whether it be sculpting or even painting faces for my fellow members. One of my biggest accomplishments as VP of Art Club was organizing my club to help paint the backdrop for my school's annual Asian Night performance. This summer, I applied to Project Success to further my experience in the field of science and to see if this is what I am truly passionate about. I want to help others and society as a whole to improve the quality of life for all. Whether be up close and personal as a physician or an analyst to find the cause of an epidemic, I want to be involved with the improvement. I plan on majoring in Biology and minoring in a social science in college. As for after college, I may attend Graduate school or Medical School but when it comes to the future, I am open minded about the opportunities yet to come. I am also a huge supporter for equal rights for all people and will not hesitate to fight for what I believe in. I am passionate about this touchy topic because I believe that every human being is equal and any societal limitation to a person's dreams should be derailed because it only slows down the progressiveness that is slowly forming in the 21st century.
Fun facts about me:1. I was born in China and immigrated to the US at the age of 3.2. My local Starbucks barista knows my order by heart.3. I can pick up both large and minuscule items in chopsticks.
My lab is located in the Boston Children's Hospital Enders Research Building. I am studying in the Luo Laboratory where we study the immune system, specifically, neutrophils that organisms create. Neutrophils are the most abundant cell type among the white blood cells and defends against invading pathogens. The goal of our research is analyze and elucidate the molecular mechanisms that control neutrophil functions. For example, Interleukin 1ß is a cytokine in the immune system that attracts neutrophils to areas with cuts as a means to protect the body from infections. Our research focuses on identifying the cause of directed movement of neutrophils during inflammation, neutrophil spontaneous cell death, neutrophil balance under both normal conditions and inflammatory conditions, researching preventative treatments for neutropenia related pneumonia by enhancing the neutrophil functions, and improvement of neutrophil functions.

My project involves the purification of the Interleukin 1ß (IL-1ß) protein from the attached His-tag. This protein is a recombinant protein and my project involves the last step of creating a recombinant protein; purification. I purified my protein by using a SUMO protease to cleave the SUMO protein that attaches the His-tag and the target protein. I would then utilize Ni-NTA agarose beads to separate the His-tag and the target protein. With the target protein, I would then be able to run tests to find the concentration and size. I could then run a SDS-Page by running the digested protein and the protein before digestion down a running gel to compare size. Utilizing a ladder, I found the molecular weight and size of the before protein which was 31 kD and and the after protein which was 17 kD. I could then also run a BCA Protein Assay to find the unknown concentration of the IL-1ß. The standard curve created by the optical density reading of the BCA protein assay was utilized to find the concentration of the IL-1ß protein. The concentration of the protein averaged at 212.25 ug/ml. In conclusion, the protein was successfully cleaved as shown by the electrophoresis with a concentration of 212.25 ug/ml.