Hello everyone! My name is Melissa. I am originally from Haiti but I've been residing in Boston for approximately seven years. I am currently a rising sophomore at Colby College in Waterville, Maine. I graduated from the John D. O'Bryant School of Math and Science last year. During the school at Colby, I work as a librarian assistant. My ultimate goal after college is to attend medical school. Currently i am majoring in French Studies and Biology while following a pre-medical track.
Fun Facts about me:
I have curly eye lashes
During my spare time at school, I enjoy making objects at the pottery club
I ran track in High School for three years
Introduce This year I am working in Luo Lab at Children's Hospital. Under Dr. Robert Luo, many researchers and graduate students conduct different experiments that correlates with Innate immunity.
My project is to investigate what affects the secretion of Interleukin 1 beta.
Question for Dr. Martin
I know you see ambition as a good thing, do you think ambition can be malevolent as well?
BCA Method
In order to answer the questions posed above, several procedures were followed. Sample Raw 264.7 cells were stimulated with LPS (I2), LPS and APT (I3) as an unstimulated sample (I1). After the cells were plated and stimulated, a cell lysate was performed. After the cell lysis, the intracellular and the secreted fluid were collected. BCA was utilized to quantify the intracellular protein in order to perform western blot. Using a standard albumin solution, a set of 50 µL dilution ranging from 2000µg/mL to 0µg/mL was prepared. 10µL of sample and standard solution was added to the micro-plates. 200 µL of the working reagent which contained A and B reagent in a ratio 50:1 was added also to the micro-plates. This was followed by 30 minutes incubation at 37 degrees Celsius then the absorbance of the samples and standard solution was measure using a spectrophotometer at 562nm. Afterward the standard solution was utilized to create a standard curve.

Assignment: Reading Summary
Inositol 1,3,4,5-tetrakisphosphate negatively regulates chemoattractant-elicited PtdIns(3,4,5)P3 signaling in neutrophils- PtdIns(3,4,5)P3 is a type of inositol phosphate that is found in many animals and plant tissues. It was found that PtdIns(3,4,5)P3 plays a key role in helping the neutrophils’ functions. PtdIns(3,4,5)P3 meticulously monitors protein translocation. By manipulating other inositol phosphates molecules such as PtdIns(1,3,4,5)P4, it was determined that the concentration is not the only mechanism used to control PtdIns(3,4,5)P3. This new knowledge about how PtdIns(3,4,5)P3 is regulated can potentially be used for therapeutic practices.

Note from Melissa: Welcome to your Project Success homepage! We've matched you with Christie Zhang in the lab of Dr. Hongbo Luo. They've suggested an article for you to read in preparation for the summer: please read it before you get here:
Inositol 1,3,4,5-tetrakisphosphate negatively regulates chemoattractant-elicited PtdIns(3,4,5)P3 signaling in neutrophils
We've also matched you up with another mentor outside of the lab to help support you through the summer and give you further exposure to science careers. Your mentor is Olivia Tawa.